The Lipid and Protein Staining Properties of Sudan II, III and IV and Their Components

Henn Kutt Neurological Service of the Second (Cornell) Medical Division, Bellevue Hospital, New York, NY; Department of Medicine, Cornell University Medical College, New York, NY

David Lockwood Neurological Service of the Second (Cornell) Medical Division, Bellevue Hospital, New York, NY; Department of Medicine, Cornell University Medical College, New York, NY

Fletcher McDowell Neurological Service of the Second (Cornell) Medical Division, Bellevue Hospital, New York, NY; Department of Medicine, Cornell University Medical College, New York, NY

Abstract

Saturated solutions of commercial Sudan II, III and IV in 60% ethanol were found to stain heat-coagulated fat-free purified human serum albumin and defatted whole serum. The protein staining components were isolated and identified by means of paper chromatography on mineral oil-impregnated filter paper with 95% ethanol as the developing agent. The brownish and yellowish fractions which migrated rapidly in this system stained proteins well, whereas the red, pink and orange fractions, which migrated slowly, stained lipids only. The solubility and staining power of the slowly migrating lipid coloring fractions remained satisfactory in absence of the rapidly moving protein staining fractions.

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The Lipid and Protein Staining Properties of Sudan II, III and IV and Their Components

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