Improved Preparation of Polyvinyl Alcohol-HgCl₂ Fixative Used for Fecal Smears

Abstract

Rather than dissolve polyvinyl alcohol (PVA) powder in a modified Schaudinn's fixative (containing glycerol) by prolonged heating and stirring, it was found better to coat the particles of PVA with glycerol first. PVA powder (Delkote brand), 5 gm, mixed with 1.5 ml of glycerol and added to 62.5 ml of distilled water, is allowed to soak 3–16 hr in a stoppered flask, then heated to 70–75 C in a water bath (with swirling) for about 10 min or until solution is complete. A separate solution of the other ingredients: 95% ethanol, 31 ml; HgCl₂ 4.5 gm; and glacial acetic acid, 5 ml, is then added to the heated PVA solution, and swirled in the stoppered flask until the mixture becomes clear. This fixative is easy to make, produces little or no sediment, does not thicken with age, and has a long shelf life. It is strongly adhesive and fixes protozoa as well or better than that made by the older method or purchased ready made.