Membrane Partitioning of the Rose-Type Chamber for the Study of Metabolic Interaction Between Different Cultures

U. Batzdorf Department of Surgery/Neurosurgery, UCLA School of Medicine, and the Brain Research Institute, Los Angeles, California, 90024

R. S. Knox Department of Surgery/Neurosurgery, UCLA School of Medicine, and the Brain Research Institute, Los Angeles, California, 90024

S. M. Pokress Department of Surgery/Neurosurgery, UCLA School of Medicine, and the Brain Research Institute, Los Angeles, California, 90024

J. C. Kennady Department of Surgery/Neurosurgery, UCLA School of Medicine, and the Brain Research Institute, Los Angeles, California, 90024

Abstract

The Rose tissue culture chamber has been divided into 2 compartments by using half-thickness gaskets with an interposed Nuclepore (General Electric Co.) filter. This permits study of the interaction of 2 different cell populations on each side of the membrane. Radioiodinated serum albumin concentration studies demonstrate rapid distribution of labelled protein across the membrane while its pore size of 0.5 μ or 0.8 μ prevents migration of the cells themselves. Equilibrium between plain medium and radioactive medium was established in 7–9 hr.

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Membrane Partitioning of the Rose-Type Chamber for the Study of Metabolic Interaction Between Different Cultures

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Membrane Partitioning of the Rose-Type Chamber for the Study of Metabolic Interaction Between Different Cultures

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