Sodium Acetate Pretreatment as an aid to Staining Endospore Nuclei of Protomyces Inundatus

Abstract

Endospore nuclei of Protomyces inundatus may be stained satisfactorily with either acetic orcein or Giemsa after the following treatment: 1.5% sodium acetate, 12 hrs, 1:3 acetic alcohol 2–4 days, wash in absolute alcohol, transfer through 1:1 alcohol-chloroform to chloroform for 30 min and return to water via graded series of chloroform-alcohol and alcohol-water.