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Abstract
Sagittal whole-body sections of frozen mice were cut on a hydraulicly driven microtome in a cryostat at -15 C by applying cotton or nylon-backed adhesive tape to the mouse before cutting. Section thickness was 20 μ The sections, still adhering to the tape, were dried in the cryostat (- 15C) under atmospheric pressure. After autoradiography, the sections were pressed to a glass slide spread with a mixture of albumin and glycerin. The slide was immersed in xylene at 30 C for 15 min. The tape was then removed from the slide, where the section remained to be stained with hematoxylin-cosin. The section thus obtained enabled the tissuc histology to h related to the autoradiogram. This method may also be applied to histochemical studies of substances insoluble in xylene.