Staining of Muscle Tissue for Quantitative Studies and Automated Morphometry

Heather M. Ráliš Department of Traumatic and Orthopaedic Surgery, Cardiff Royal Infirmary, Cardiff, CF2 ISZ, Wales; Department of Histopathology, The Children's Hospital, Western Bank, Sheffield, 10, England

D. R. Gadsdon Department of Traumatic and Orthopaedic Surgery, Cardiff Royal Infirmary, Cardiff, CF2 ISZ, Wales; Department of Histopathology, The Children's Hospital, Western Bank, Sheffield, 10, England

Z. A. Ráliš Department of Traumatic and Orthopaedic Surgery, Cardiff Royal Infirmary, Cardiff, CF2 ISZ, Wales; Department of Histopathology, The Children's Hospital, Western Bank, Sheffield, 10, England

Abstract

Seventy-six staining tests were carried out on paraffin sections of human and animal muscle to find a suitable staining method for quantitative morphometry of muscle fibers. The results were evaluated under the light microscope, on black and white photomicrographs and on an image analysing computer, the Quantimet 720. A brilliant scarlet-phosphotungstic acid-tartrazine method is described and recommended for automated morphometry after additional testing on 140 sections of developing human muscle using the Quantimet 720.

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Staining of Muscle Tissue for Quantitative Studies and Automated Morphometry

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Staining of Muscle Tissue for Quantitative Studies and Automated Morphometry

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