Histochemical Demonstration of Enzyme Activities in Plastic and Paraffin Embedded Tissue Sections

Sadanobu Higuchi Department of Environmental Health Sciences, School of Medicine, Johns Hopkins University, Baltimore, Maryland, 21205

Moritaka Suga Department of Environmental Health Sciences, School of Medicine, Johns Hopkins University, Baltimore, Maryland, 21205

Arthur M. Dannenberg Department of Environmental Health Sciences, School of Medicine, Johns Hopkins University, Baltimore, Maryland, 21205

Brian H. Schofield Department of Epidemiology, School of Hygiene and Public Health, and Department of Pathology and Orthopedic Research Laboratory, School of Medicine, Johns Hopkins University, Baltimore, Maryland, 21205

Abstract

Histochemical staining for enzymes is usually performed on frozen sections. This report lists the longer incubation times required to demonstrate esterase, acid phosphatase, β-galactosidase, and cytochrome oxidase in plastic embedded and routine paraffin embedded tissues. The sections embedded in plastic, i.e. water soluble methacrylate (Polyscience's JB-4) and cut at 2 μm, were far superior to frozen Sections and paraffin embedded sections both in tissue detail and in the localization of the histochemical reaction product.

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Histochemical Demonstration of Enzyme Activities in Plastic and Paraffin Embedded Tissue Sections

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Histochemical Demonstration of Enzyme Activities in Plastic and Paraffin Embedded Tissue Sections

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