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Abstract
It has been down that the degradation of thiazine dyes which normally occurs in methanolic solution, as in the case of Romanowsky blood stains, can be prevented by nuking the solution acidic. In a certain range of oddity, the stain precipitates in the form of monothiazine cosinate, but by making the solution sufficiently acidic, eosin is protonated and the precipitate cannot form. These observations have been used to develop a blood stain which is stable, even at elevated temperatures, for sexed months. For use the stain is neutralized by a specially formulated fixative solution.