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Stain Technology Volume 57, 1982 - Issue 2
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Original Article

Basophilic “Dark Cells:” Optimal Conditions for Toluidine Blue Staining

D. F. Parsons Ultrastructural Analysis Section, Laboratory Medicine Institute, Division of Laboratories and Research, New York State Department of Health, Albany, New York, 12201
,
S. Braun Ultrastructural Analysis Section, Laboratory Medicine Institute, Division of Laboratories and Research, New York State Department of Health, Albany, New York, 12201
&
M. Marko Ultrastructural Analysis Section, Laboratory Medicine Institute, Division of Laboratories and Research, New York State Department of Health, Albany, New York, 12201
Pages 75-78 | Published online: 12 Jul 2009
 
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Abstract

Differential staining of basophilic “dark cells” of epithelia in thick epoxy sections with toluidine blue is critically affected by fixation and embedding conditions. Optimal conditions are described.

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