The Use of Primuline to Identify the Septum Polysaccharide of the Fission Yeast Schizosaccharomyces Pombe

Abstract

Treatment of cells and purified cell walls of the fission yeast Schizosaccharomyces pombe with primuline reveals the septum as a bright fluorescent band. When polysaccharides containing (1 → 3)-β-, (1 → 6)-β- or (1 → 3)-α-glucosidic linkages are treated with primuline, only those molecules containing chains of (1 → 3)-β-glucosyl residues are stained. This implies that (1 → 3)-β-glucan is present in the septum of Schiz. pombe as the main constituent.