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Stain Technology Volume 59, 1984 - Issue 3
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Original Article

Improvement in the Specificity of the Silver Staining Technique for Ag NOR-ASSOCIATED Acidic Proteins in Paraffin Sections

Franz Thiebaut Laboratory of Developmental Biology, University of Paris-Nord, 93012, Bobigny-Cedex, France; Laboratory for Electron Microscopy and Morphometry, Department of Pathology, Norsk Hydro's Institute for Cancer Research, The Norwegian Radium Hospital, Oslo 3, Norway
,
Jean Paul Rigaut Laboratory of Developmental Biology, University of Paris-Nord, 93012, Bobigny-Cedex, France; Laboratory for Electron Microscopy and Morphometry, Department of Pathology, Norsk Hydro's Institute for Cancer Research, The Norwegian Radium Hospital, Oslo 3, Norway
&
Albrecht Reith Laboratory of Developmental Biology, University of Paris-Nord, 93012, Bobigny-Cedex, France; Laboratory for Electron Microscopy and Morphometry, Department of Pathology, Norsk Hydro's Institute for Cancer Research, The Norwegian Radium Hospital, Oslo 3, Norway
Pages 181-185 | Published online: 12 Jul 2009
 
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Abstract

Some recently developed silver staining methods allow selective staining of acidic nucleolar proteins. Pretreating deparaffinized sections with Schiffs reagent improves the specificity of Goodpasture and Bloom's AgNOR staining (as modified by Kodama et al.) after aldehyde fixation.

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