A 595 Nanometer Band-Pass Filter Enhances the Contrast of in Situ Hybridization Signals on Chromosome Observed After Biotin/Avidin-Alkaline Phosphatase Localization

Abstract

In situ hybridization with DNA probes labeled with biotin and detected by the avidin-alkaline phosphatase/5-bromo-chloroh)doxyl phosphate-nitro blue tetrazolium system has been used to localize DNA sequences in chromosomes. To observe the hybridization signals, a phase contrast microscope has often been used because of the good visibility it provides. Use of a 595 nm band pass filter with the phase contrast microscope enhances signal contrast after in situ hybridization without reducing resolution.