Observing Transmitting Tissue and Other Structures in the Pistil Using A Fluorescent Stain

Abstract

Using safranin O as a fluorescent stain at a wavelength range of 355—425 nm has allowed us to distinguish the transmitting tissue and the nature of this tissue in pistils of members of the Geraniaceae and Gentianaceae. Xylem, amyloplasts, and high tannin containing tissues, such as mericarp walls, were also readily differentiated.