Determination Of Plasma Cell Labelling Index With Bromodeoxyuridine Using A Double Immunoenzymatic Technique

Martine Ffrench Analytic Cytology Laboratory, C. Bernard University, Central Hematology and Cytogenetics Laboratory and Hematology Service, E. Herriot Hospital, Lyon, France

Jean Pierre Magaud Analytic Cytology Laboratory, C. Bernard University, Central Hematology and Cytogenetics Laboratory and Hematology Service, E. Herriot Hospital, Lyon, France

Pierre Duhaut Analytic Cytology Laboratory, C. Bernard University, Central Hematology and Cytogenetics Laboratory and Hematology Service, E. Herriot Hospital, Lyon, France

Paul Andrè Bryon Analytic Cytology Laboratory, C. Bernard University, Central Hematology and Cytogenetics Laboratory and Hematology Service, E. Herriot Hospital, Lyon, France

Jean Jacques Viala Analytic Cytology Laboratory, C. Bernard University, Central Hematology and Cytogenetics Laboratory and Hematology Service, E. Herriot Hospital, Lyon, France

Abstract

Myeloma plasma cells were double stained using peroxidase and alkaline phosphatase labelled monoclonal anti-BrdU and anti-intracytoplasmic immunoglobulins. Samples were methanol fixed; DNA was denatured with formamide. The results allowed easy identification of plasma cells, their cytological examination and the calculation of percentage of plasma cells in S phase. Good correlation was found with the labelling index obtained with tritiated thymidine.

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Determination Of Plasma Cell Labelling Index With Bromodeoxyuridine Using A Double Immunoenzymatic Technique

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Determination Of Plasma Cell Labelling Index With Bromodeoxyuridine Using A Double Immunoenzymatic Technique

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