A Modified Mallory-Cason Staining Procedure for Large Cryosections

M. B. M. van Leeuwen Department of Anatomy and Embryology, State University of Groningen, 9713, EZ, Groningen; Department of Functional Anatomy, University of Utrecht, 3512, BK, Utrecht, The Netherlands

A. J. H. Deddens Department of Anatomy and Embryology, State University of Groningen, 9713, EZ, Groningen; Department of Functional Anatomy, University of Utrecht, 3512, BK, Utrecht, The Netherlands

P. O. Gerrits Department of Anatomy and Embryology, State University of Groningen, 9713, EZ, Groningen; Department of Functional Anatomy, University of Utrecht, 3512, BK, Utrecht, The Netherlands

B. Hillen Department of Anatomy and Embryology, State University of Groningen, 9713, EZ, Groningen; Department of Functional Anatomy, University of Utrecht, 3512, BK, Utrecht, The Netherlands

Abstract

The classic Mallory-Cason staining procedure has been modified for application to sections “on tape” obtained from large deep frozen tissue specimens. These 20 μm cryosections are collected on tape from a large heavy duty cryomicrotome. The stained sections provide anatomical details that are not revealed by other techniques. The merit of this procedure is found in the support of modern medical modalities, both for research and educational purposes.

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A Modified Mallory-Cason Staining Procedure for Large Cryosections

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A Modified Mallory-Cason Staining Procedure for Large Cryosections

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