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Original Article

A Method to Determine the end Point of Decalcification of Hard Tissue and Bone

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Pages 77-83 | Published online: 12 Jul 2009
 

Abstract

A method for decalcification end point determination of mineralized tissue is described. The calcium content of the decalcification solution was determined colorimetrically with a “continuous automatic analyzer” with a high degree of accuracy. The end point method used has been tested on two decalcification methods, 5% nitric acid with or without ultrasonic treatment. The results suggest it is possible to quantitate the decalcification process.

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