Silver Enhancement of Colloidal Gold Particles in Deplasticized Semithin Epoxy Sections

Abstract

A simple method for light microscope screening of pre-embedded, gold-labeled samples, by silver enhancing deplasticized semithin epoxy sections is presented. This method is of great help in visualizing the topography of gold labeled sites at low magnification and for selecting labeled sample regions for subsequent electron microscope analysis on serial ultrathin sections. The method was developed during a study of lectin binding sites on the egg surface of the anuran amphibian Discoglossus pictus

Key Words:

• gold labeling
• pre-embedding
• silver enhancement
• deplasticized sections
• biotinylated lectins
• streptavidin-gold
• amphibian egg