Preparing Mixed Cellulose Ester or Polycarbonate Membrane Filter Replicas for Transmission Electron Microscopy

Abstract

Simple methods for preparing large numbers of grids exhibiting excellent coverage of intact replicas on mixed cellulose ester or polycarbonate membrane filters are described. The techniques ensure that grids and carbon replicas receive identical treatment and are not rearranged or lost during processing. The techniques permit grids and filter sections to be handled en masse rather than individually. Also, replica section squares remain centered on the grids. A temporary grid storage method (“grid- pad”) is also described, which facilitates grid identification and handling.

Key Words:

• Replicas
• cellulose ester filters
• polycarbonate filters
• grid washing