Enhanced Polymer One-Step Staining for Proliferating Cell Nuclear Antigen in Squamous Cell Carcinoma of the Tongue

Kazuo Sano Second Department of Oral and Maxillofacial Surgery, Nagasaki University School of Dentistry, 1-7-1, Sakamoto, Nagasaki, 852, Japan

Joji Sekine Second Department of Oral and Maxillofacial Surgery, Nagasaki University School of Dentistry, 1-7-1, Sakamoto, Nagasaki, 852, Japan

Tsugio Inokuchi Second Department of Oral and Maxillofacial Surgery, Nagasaki University School of Dentistry, 1-7-1, Sakamoto, Nagasaki, 852, Japan

Michael B. Pe Second Department of Oral and Maxillofacial Surgery, Nagasaki University School of Dentistry, 1-7-1, Sakamoto, Nagasaki, 852, Japan

Guowu Ma Second Department of Oral and Maxillofacial Surgery, Nagasaki University School of Dentistry, 1-7-1, Sakamoto, Nagasaki, 852, Japan

Abstract

A rapid enhanced polymer one-step staining for proliferating cell nuclear antigen [EPOS-PCNA) in formalin fixed, paraffin embedded sections of tongue squamous cell carcinoma is described. EPOS-PCNA was compared with PCNA immunohistochemistry using the avidin-biotin complex (ABC) method, with or without autoclave pretreatment. Significant correlation of PCNA labeling index (percentage of labeled cells/total cells counted) was observed between EPOS-PCNA and the immunohistochemical protocol using autoclave pretreatment. We consider this method a reliable, timesaving technique that would be useful in quantitative histopatbology and in performing double immunostaining.

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Enhanced Polymer One-Step Staining for Proliferating Cell Nuclear Antigen in Squamous Cell Carcinoma of the Tongue

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Enhanced Polymer One-Step Staining for Proliferating Cell Nuclear Antigen in Squamous Cell Carcinoma of the Tongue

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