Fluorescence in Situ Hybridization (FISH): DNA Probe Production and Hybridization Criteria

Abstract

We describe methods for the production of fluorescence in situ hybridization (FISH) probes and the utilization of these probes for the detection of complementary DNA sequences with accuracy and sensitivity for application in both basic research and clinical diagnosis. Due to the frequent use of FISH in many laboratories, it is important to apply the most convenient and reproducible approach. This review describes some of the most recent techniques, and includes versatile, effective and simple methods of probe production and fluorescence in situ hybridization. We also describe methods for the production of region-specific and chromosome-specific DNA probes and hybridization techniques for the visualization of these probes.

Key Words:

• complementary sequences
• DNA probes
• FISH
• fluorescence in situ hybridization
• probe production
• review