Effect of Peroxynitrite Scavenging on Endothelial Cells Stimulated by Plasma from Women with Preeclampsia: A Proteomic Approach

Abstract

Hypothesis: Increased peroxynitrite formation in endothelial cells incubated with plasma from women with preeclampsia alters the expression of specific endothelial proteins. Methods: We used a proteomic approach to examine the effect of a peroxynitrite scavenger (FeTPPS, 5 μmol/L) on the expression of endothelial proteins in response to plasma from women with preeclampsia. After treatment, cells were subjected to two-dimensional gel electrophoresis and proteins were identified using mass spectrometry. Results: Peroxynitrite scavenging decreased the expression of structural proteins, cytokeratin, actin-γ-1, and moesin, and the calcium-binding protein, calumenin. Conclusions: This study has identified novel proteins that are regulated by peroxynitrite in response to plasma from women with preeclampsia.

• Preeclampsia
• Oxidative Stress
• Peroxynitrite
• Endothelium

ACKNOWLEDGMENTS

This work was supported by grants from the Canadian Institutes for Health Research (CIHR). SS was supported by the Maternal-Fetal-Newborn Health and Strategic Training Initiative in Research in Reproductive Health Sciences Training Programs of the CIHR, the Alberta Heritage Foundation for Medical Research (AHFMR), and the Junior Personnel Award from the Heart and Stroke Foundation of Canada. STD is an AHFMR Scientist and a Canada Research Chair in Women's Cardiovascular Health.

Declaration of Interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.