Changes in protein and gene expression of angiotensin II receptors (AT₁ and AT₂) in aorta of diabetic and hypertensive rats

Abstract

Diabetes and hypertension have been associated with cardiovascular diseases and stroke. Some reports have related the coexistence of hypertension and diabetes with increase in the risk of developing vascular complications. Recently some studies have shown results suggesting that in the early stages of diabetes and hypertension exist a reduced functional response to vasopressor agents like angiotensin II (Ang II), which plays an important role in blood pressure regulation mechanism through the activation of its AT₁ and AT₂ receptors. For that reason, the aim of this work was to study the gene and protein expression of AT₁ and AT₂ receptors in aorta of diabetic SHR and WKY rats. Diabetes was induced by the administration of streptozotocin (60 mg/kg i.p.). After 4 weeks of the onset of diabetes, the protein expression was obtained by western blot and the mRNA expression by RT-PCR. Our results showed that the hypertensive rats have a higher mRNA and protein expression of AT₁ receptors than normotensive rats while the AT₂ expression remained unchanged. On the other hand, the combination of diabetes and hypertension increased the mRNA and protein expression of AT₁ and AT₂ receptors significantly. In conclusion, our results suggest that diabetes with hypertension modifies the mRNA and protein expression of AT₁ and AT₂ receptors. However, the overexpression of AT₂ could be associated with the reduction in the response to Ang II in the early stage of diabetes.

• Hypertension
• diabetes
• AT1 receptor
• AT2 receptor
• Renin Angiotensin System (RAS)

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article. This work was supported by grants CONACYT CB-1012-01-183660 (Consejo Nacional de Ciencia y Tecnología) CONACYT, project SIP-IPN-20150991 (Secretaría de Investigación y Posgrado del Instituto Politécnico Nacional) and CONACYT scholarship 356818 assigned to the student Rodrigo Romero Nava. The authors thank to Contreras K, Soto E, Nielsen N, Lugo G, Gutierréz R and Robles D for their technical assistance.