Production of Angiotensinogen by Cultured Rat Aortic Smooth Muscle Cells

P. Eggena Renin Biochemistry Laboratory Veterans Administration Medical Center, 16111 Plummer Street, Sepulveda, CA, 91343; Department of Medicine, University of California School of Medicine, Los Angeles, CA, USA

F. Krall Renin Biochemistry Laboratory Veterans Administration Medical Center, 16111 Plummer Street, Sepulveda, CA, 91343; Department of Medicine, University of California School of Medicine, Los Angeles, CA, USA

M. P. Eggena Renin Biochemistry Laboratory Veterans Administration Medical Center, 16111 Plummer Street, Sepulveda, CA, 91343; Department of Medicine, University of California School of Medicine, Los Angeles, CA, USA

K. Clegg Renin Biochemistry Laboratory Veterans Administration Medical Center, 16111 Plummer Street, Sepulveda, CA, 91343; Department of Medicine, University of California School of Medicine, Los Angeles, CA, USA

M. Fittingoff Renin Biochemistry Laboratory Veterans Administration Medical Center, 16111 Plummer Street, Sepulveda, CA, 91343; Department of Medicine, University of California School of Medicine, Los Angeles, CA, USA

J. D. Barrett Renin Biochemistry Laboratory Veterans Administration Medical Center, 16111 Plummer Street, Sepulveda, CA, 91343; Department of Medicine, University of California School of Medicine, Los Angeles, CA, USA

Abstract

This study was conducted to further investigate angiotensinogen synthesis in rat aortic smooth muscle cells (SMC) grown in culture. Tissue cultures maintained in defined medium neither grew nor synthesized angiotensinogen. However, in the presence of 5% homologous serum both cell proliferation and angiotensinogen synthesis became apparent. Substitution of normal control serum with that of bilaterally nephrectomized rats or animals given dexamethasone (10mg/kg, ip) led to a further significant increase in angiotensinogen production. In contrast, serum from adrenalectomized rats suppressed angiotensinogen synthesis below the rate observed with normal serum. A positive linear correlation (r=0.96, p0.01) was evident between the serum angiotensinogen level and the rate of de novo synthesis of this protein. No correlations were found between cell proliferation and either angiotensinogen synthesis or serum angiotensinogen levels. Dexamethasone added to serum did not stimulate the rate of angiotensinogen synthesis and appeared to inhibit cell proliferation. Stimulation or suppression of angiotensinogen synthesis was not accompanied by a statistically significant change in angiotensinogen specific mRNA. The data indicate a complex regulation of angiotensinogen in vascular smooth muscle cells in culture.

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Production of Angiotensinogen by Cultured Rat Aortic Smooth Muscle Cells

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Production of Angiotensinogen by Cultured Rat Aortic Smooth Muscle Cells

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