Abstract
The study was aimed at examining the effects of tert-butyl hydroperoxide (tBHP) on hepatic transcriptome expression patterns of the teleost fish Lithognathus mormyrus. tBHP is an organic hydro-peroxide, widely used as a model pro-oxidant. It generates the reactive oxygen species (ROS) tert-butoxyl and tert-butylperoxyl. Complementary DNAs of tBHP-treated vs control fish were applied onto a previously produced cDNA microarray of ∼ 1500 unique sequences. The effects of the tBHP application were demonstrated by leukocyte infiltration into the liver and by differential expression of various genes, some already known to be involved in ROS-related responses. Indicator genes of putative ROS effects were: aldehyde dehydrogenase 3A2, Heme oxygenase and the hemopexin-like protein. Putative indicators of transendothelial leukocyte migration and function were: p22phox, Rac1 and CD63-like genes. Interestingly, 7-dehydrocholesterol reductase was significantly down-regulated in response to all treatments. Several non-annotated genes revealed uniform directions of differential expression in response to all treatments.
Acknowledgements
Dr Miriam Kott-Gutkowski from the Hebrew University of Jerusalem is thanked for provision of slide-related services. The ROS-related advice of Professor A. Reznick of the Technion, Haifa and the bio-informatic assistance of Dr Michael Shmoish from the Technion Bioinformatics Knowledge Unit of the Lokey Interdisciplinary Center for Life Sciences and Engineering, Haifa, is highly appreciated.
Declaration of interest: The study was supported by the EU projects GENIPOL (EVK3-CT-2001-00057) and MARINEGENOMICS EUROPE (Contract 505403). M.A. was a recipient of the Yohai Ben-Nun and Rieger foundation and The Technion scholarships. The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.
This paper was first published online on Early Online on 21 June 2010.