The Reaction of Sulfite Radical Anion with Nucleic Acid Components

Abstract

The sulfite radical anion (SO₃⁻) is the first intermediate in the autoxidation of sulfite to sulfate. Using competition kinetics, its reactivities with the nucleic acid bases and the corresponding nucleosides were investigated. The second order rate constants were found to be rather low, k < 1 × 10⁶dm³mol⁻¹s⁻¹ at pH 7. As a competitor, the carotenoid crocin was used, which was found to be bleached very efficiently by SO₃⁻ (k = 1.0 × 10⁹dm³mol⁻¹S⁻¹).

Key Words:

• Sulfite radical
• nucleic acid bases
• nucleosides
• crocin
• pulse radiolysis
• rate constants
• competition studies