Limited Protection Against Iron-Induced Lipid Peroxidation by Cord Blood Plasma

Abstract

The ability of plasma from newborn babies (cord blood) and adults to inhibit iron-induced lipid peroxidation was compared. The caeruloplasmin and transferrin concentrations, and latent iron-binding capacity were lower in the babies (p < 0.001). The plasma of many of the babies had no latent iron-binding capacity and contained non-protein-bound iron (measured by the bleomycin assay). The in vitro ability of plasma to inhibit iron-induced liposome peroxidation by either ferroxidase antioxidant activity (caeruloplasmin) or iron-binding antioxidant activity (transferrin) was measured. The antioxidant activity in both assays was decreased in the babies (p < 0.001). The percentage inhibiton of peroxidation in the iron-binding antioxidant assay correlated positively with the latent iron-binding capacity (p < 0.001) and negatively with the presence of bleomycin-detectable iron (p < 0.02) in the babies. This assay produced stimulation of peroxidation in 42% of the babies but none of the adults. The diminished capacity of cord blood plasma to prevent iron-induced lipid peroxidation may predispose the newborn baby to the toxic effects of oxygen.

Key Words:

• newborn
• transferrin
• caeruloplasmin
• bleomycin-detectable iron
• lipid peroxidation