Effect of Ozone on the Activities of Reactive Oxygen Scavenging Enzymes in Rbc of Ozone Exposed Japanese Charr (Salvelinus Leucomaenis)

Abstract

The effect of ozone exposure on the activities of reactive oxygen scavenging enzymes (SOD†, catalase, GSH-Px) in RBC of Japanese charr (Salvelinus leucomaenis) was examined. Ozone (0, 0.4 and 0.7 ppm as initial concentrations) was exposed to Japanese charr for 30 min, which definitely caused serious membrane damage to RBC of fish. Ozone exposure at 0.4 and 0.7 ppm decreased activities of both catalase and GSH-Px by 80 to 57+ of the control. On the other hand, the activities of SOD remained unaffected even by 0.7 ppm ozone exposure. A hypothesis on the RBC membrane damage and participation of SOD and heme-iron was proposed.

Key Words:

• Ozone
• Japanese charr
• reactive oxygen scavenge enzymes
• SOD
• erythrocyte
• membrane damage