Application of Methionine as a Detector Molecule for the Assessment of Oxygen Radical Generation by Human Neutrophils and Endothelial Cells

Abstract

Diverse cell types can generate reactive oxygen species (ROS) which are implicated in many disease processes and are ascribed both beneficial and deleterious roles. In vitro studies of this phenomenon indicate that properties of the microenvironment in culture influence the cells' behaviour with regard to ROS generation in vivo. To date, however, the assessment of cellular ROS generation has been limited to techniques which are invasive of the culture environment, or require cells to be in suspension. This study describes the application of NMR spectroscopy to the detection of ROS generation, a technique which is non-invasive of the cell culturing environment.

Key Words:

• NMR spectroscopy
• neutrophil free radical generation
• endothelial cell free radical generation
• methionine
• cell culture