Stimulation of Mitochondrial Oxygen Consumption in Isolated Cardiomyocytes After Hypoxia-Reoxygenation

Abstract

An increase in mitochondrial matrix free calcium has been shown to occur during oxygen and substrate deprivation of the perfused heart which predisposes to calcium overload and inhibition of mitochondrial function on reoxygenation. In the current study we have assessed the effect of substrate free hypoxia on mitochondrial oxygen consumption and ATP synthesis in isolated rat cardiomyocytes. Myocytes were subjected to 40 min of substrate-free hypoxia and the oxygen consumption measured together with the effects on ATP and PCr synthesis. After hypoxia myocytes showed a fall in ATP to 10%of the control value. Within 5 sec of reoxygenation the ATP level recovered to a new steady state level of 30% of the original value. The rate of oxygen consumption of hypoxic/reoxygenated cells was 3–4 fold higher than that of cells maintained under normoxic controls but in the presence of oligomycin the difference was only 1.5-fold higher, indicating a greater requirement for mitochondrial synthesis of ATP following reoxygenation. Reoxygenation in the absence of extracellular Ca²⁺ resulted in a lower rate of oxygen consumption (50% of the rate measured in the presence of 1 mM-Ca²⁺) but did not affect the steady state concentration of ATP attained 5 min after reoxygenation. These results support the idea that the increased 0₂ consumption of myocytes following hypoxia/ reoxygenation is due to an increased demand for ATP synthesis by mitochondria and is a response to the Na⁺ and Ca²⁺ loading of the cells which occurs under these conditions. This increased demand is likely to result in a greater generation of free radicals such as superoxide by the respiratory chain which could impair cellular function over the long term.

Key Words:

• cardiomyocytes
• reperfusion damage
• mitochondria
• hypoxia-reoxygenation