Design of a novel gut bacterial adhesion model for probiotic applications

Laetitia RodesBiomedical Technology and Cell Therapy Research Laboratory, Faculty of Medicine, Department of Biomedical Engineering and Artificial Cells and Organs Research Centre, McGill University, Duff Medical Building, Montreal, Quebec, Canada

Michael Coussa-CharleyBiomedical Technology and Cell Therapy Research Laboratory, Faculty of Medicine, Department of Biomedical Engineering and Artificial Cells and Organs Research Centre, McGill University, Duff Medical Building, Montreal, Quebec, Canada

Daniel MarinescuBiomedical Technology and Cell Therapy Research Laboratory, Faculty of Medicine, Department of Biomedical Engineering and Artificial Cells and Organs Research Centre, McGill University, Duff Medical Building, Montreal, Quebec, Canada

Arghya PaulBiomedical Technology and Cell Therapy Research Laboratory, Faculty of Medicine, Department of Biomedical Engineering and Artificial Cells and Organs Research Centre, McGill University, Duff Medical Building, Montreal, Quebec, Canada

Marc FakhouryBiomedical Technology and Cell Therapy Research Laboratory, Faculty of Medicine, Department of Biomedical Engineering and Artificial Cells and Organs Research Centre, McGill University, Duff Medical Building, Montreal, Quebec, Canada

Sana AbbasiBiomedical Technology and Cell Therapy Research Laboratory, Faculty of Medicine, Department of Biomedical Engineering and Artificial Cells and Organs Research Centre, McGill University, Duff Medical Building, Montreal, Quebec, Canada

Afshan KhanBiomedical Technology and Cell Therapy Research Laboratory, Faculty of Medicine, Department of Biomedical Engineering and Artificial Cells and Organs Research Centre, McGill University, Duff Medical Building, Montreal, Quebec, Canada

Catherine Tomaro-DuchesneauBiomedical Technology and Cell Therapy Research Laboratory, Faculty of Medicine, Department of Biomedical Engineering and Artificial Cells and Organs Research Centre, McGill University, Duff Medical Building, Montreal, Quebec, Canada

Satya PrakashBiomedical Technology and Cell Therapy Research Laboratory, Faculty of Medicine, Department of Biomedical Engineering and Artificial Cells and Organs Research Centre, McGill University, Duff Medical Building, Montreal, Quebec, CanadaCorrespondence[email protected]

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Abstract

A new gut bacterial adhesion model has been developed. For this, a continuous-flow bioreactor packed with bacteria-coated beads was designed to simulate the gut lining and other features. In vitro model efficacy shows successful bacterial cell gut adhesions: bacterial adhesion was higher with mucin–alginate compared to controls. In feasibility study, adhesion of Lactobacillus fermentum NCIMB 5221 and Lactobacillus reuteri NCIMB 701359 was investigated for their metabolic activities for bile salt. Bile salt hydrolase (BSH)-active Lactobacillus reuteri exerted higher activity than non-BSH-active L. fermentum. This model has potential use in gut health, probiotic, bacterial cell gut adhesion and other delivery applications.

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Design of a novel gut bacterial adhesion model for probiotic applications

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Design of a novel gut bacterial adhesion model for probiotic applications

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