Giant vesicles as encapsulating matrix for stabilizing alcohol oxidase and as container for coupled enzymatic reactions

Abstract

Encapsulation of a multimeric alcohol oxidase (AOx) in giant vesicles (GVs) was prepared by rapid evaporation method. The specific activity (477 U/mg-protein) of the GV encapsulated AOx was 3.5 fold higher than the free AOx. The half-life (˜59.4 hours) of the encapsulated AOx was 20-fold higher than the free enzyme at 30°C. Further, the GV was used to encapsulate AOx and HRP together as coupled reaction catalyst for chromogen-based optical detection of alcohols. The results confirmed potential application of the GV as micro-dimensional container for enhancing stability, activity and biosensing applications of AOx.

Keywords::

• ABTS
• alcohol
• alcohol oxidase
• coupled reaction
• giant vesicles