![Sample our Bioscience journals, sign in here to start your access, Latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5925/TOC-Subject-Sample-2021-Bioscience.jpg"})
Abstract
Regenerative medicine deals with the possible use of stem cells to repair tissues damaged by aging and related diseases, including amyloidoses. In the latter case, the toxicity of the amyloid deposits can, in principle, question the possibility to graft specific tissues by undifferentiated cells. To assess whether stem cells are vulnerable to amyloid toxicity, we exposed, in culture, murine embryonic stem (ES) cells and haematopoietic progenitor (HP) cells to oligomers of the amyloidogenic peptide Aβ42 at concentrations previously shown to be cytotoxic to several other cell types. These stem cells did not display any sign of apoptosis and their survival, proliferation and differentiation were not affected by the oligomers although the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay revealed that ES, but not HP, cells displayed some impaired ability to reduce the tetrazole salts possibly as a result of transient oxidative stress. Our results support a remarkable resistance of the investigated stem cells against amyloids and hence their potential use in cell therapy of Alzheimer's disease and, possibly, other amyloid diseases.
| Abbreviations | ||
| TEM | = | transmission electron microscopy |
| ES | = | murine embryonic stem cells |
| HP | = | haematopoietic progenitor cells |
| MTT | = | 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide |
| AFM | = | atomic force microscopy |
| LDPBC | = | light density peripheral blood cells |
| LDH | = | lactate dehydrogenase |
| PI | = | propidium iodide |
| Abbreviations | ||
| TEM | = | transmission electron microscopy |
| ES | = | murine embryonic stem cells |
| HP | = | haematopoietic progenitor cells |
| MTT | = | 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide |
| AFM | = | atomic force microscopy |
| LDPBC | = | light density peripheral blood cells |
| LDH | = | lactate dehydrogenase |
| PI | = | propidium iodide |
Acknowledgements
This work was supported by: the Italian MIUR (FIRB Project N. RBIP06FH7J, PRIN Project N. 2007XY59ZJ), Fondazione CARIPLO (Projects 2007.5151and 2009-2543), UNIPV-Regione Lombardia (Project on Material Science and Biomedicine), Fondazione I.R.C.C.S. Policlinico San Matteo, Millipore.