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Amyloid
The Journal of Protein Folding Disorders
Volume 2, 1995 - Issue 3
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Original Article

The carbohydrate structure of the amyloid immunoglobulin light chain protein EPS

Lone Aanerud Omtvedt Biotechnology Centre of Oslo, University of Oslo, P.O. Box 1125 Blindem, N-0316, Oslo, NorwayCorrespondence[email protected]
,
Svein Haavik Department of Pharmacognosy, Institute of Pharmacy, P.O. Box 1068 Blindem, N-0316, Oslo, Norway
,
Elizabeth F. Hounsell Department of Biochemistry and Molecular Biology, University College, Gower Street, London, UK
,
Hilde Barsett Department of Pharmacognosy, Institute of Pharmacy, P.O. Box 1068 Blindem, N-0316, Oslo, Norway
&
Knut Sletten Biotechnology Centre of Oslo, University of Oslo, P.O. Box 1125 Blindem, N-0316, Oslo, Norway
Pages 150-158 | Received 10 Feb 1995, Published online: 06 Jul 2009
 
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Abstract

The glycosylated amyloid light chain protein EPS was isolated from amyloid fibrils from the liver of a patient with Waldenstrom's macroglobulinemia. A tryptic glycopeptide of EPS (200 nmol) was isolated and characterized by 1H-n. m. r. The glycopeptide was digested with N-glycanase and the released oligosaccharides analyzed by high performance anion exchange chromatography. The monosaccharide composition and the linkage of the different oligosaccharides were determined by methanolysis and methylation analysis. The NMR spectrum revealed a bisected biantennary oligosaccharide as the main structure. HPAEC-PAD demonstrated the presence of several other related glycans in the glycopeptide preparation.

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