A quantitative approach to evaluate urinary benzene and S-phenylmercapturic acid as biomarkers of low benzene exposure

Abstract

Context: Benzene is a ubiquitous pollutant; smoking habit, genetic polymorphisms, and analytical difficulties impact the identification of the best biomarker.

Objective: To apply a systematic quantitative approach to evaluate urinary benzene (BEN-U) and S-phenylmercapturic acid (SPMA) as biomarkers of low benzene exposures.

Methods: Seventy-one blue collar refinery workers, 97 white collar refinery workers and 108 general population subjects were included. Intrinsic characteristics, sampling and analytical issues were compared.

Results: BEN-U and SPMA were detected in 99% and 78% of samples, which correlated with benzene exposure (r = 0.456 and r = 0.636, respectively) and with urinary cotinine (r = 0.630 and r = 0.570, respectively). Intrinsic characteristics were similar for the two biomarkers: specificity (0.64 and 0.69 for BEN-U and SPMA), sensitivity (0.74 and 0.83), as well as intra- and inter-individual variability (150% and >14 for both).

Conclusion: BEN-U and SPMA show similar intrinsic characteristics; analytical issues in detecting SPMA suggest that BEN-U is more convenient for investigating low exposure levels.

Keywords::

• Environmental pollution/Ecotoxicology
• mass spectroscopy
• chemical carcinogenesis

Acknowledgments

We are indebted to the subjects who volunteered for the study.

Declaration of interest

The authors thank AISPO for financial support.