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Abstract
We developed a specific hybridization assay for direct detection of long non-coding RNA urothelial carcinoma associated-1 (lncRNA-UCA1). Total RNA was extracted from urine pellet samples (bladder carcinoma patients and controls). Then, we compared the developed nanoassay with quantitative real time polymerase chain reaction (qRT-PCR) results in detection of urine UCA1 in bladder cancer and control samples. The sensitivity and the specificity of UCA1 nanoassay were 92.1% and 93.3%, respectively. The concordance of the two methods was 98%. Interestingly, all bilharzial benign cases showed negative lncRNA-UCA1 using both methods. UCA1-nanoassay is a valid test for direct detection of urine UCA1 for bladder cancer detection.
Acknowledgements
The authors acknowledge Dr. Nahla Awad, at the early cancer detection unit, Faculty of Medicine, Ain Shams University, for her help in the cytological examination of urine samples and Dr. Ahmed I. Nosseir at Misr University for Science and Technology for his help in preparing the gold nanoparticles.
Declaration of interest
This work was supported by Ain Shams University Research Projects 2014-15.
The authors declare that they have no competing interests.
Supplementary material available online
Supplementary Table 1 and 2, Figures 1a and 1b