Urinary 5-hydroxymethyluracil and 8-oxo-7,8-dihydroguanine as potential biomarkers in patients with colorectal cancer

Abstract

Context: Oxidative stress linked with chronic inflammation is associated with etiology of the colorectal cancer.

Objectives: To assess the diagnostic utility of urinary excretion of oxidatively modified DNA bases/nucleoside: 8-oxo-7,8-dihydroguanine (8-oxoGua), 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo) and 5-hydroxymethyluracil (5-hmUra).

Materials and methods: Seventy-two healthy controls, 15 patients with adenomas and 56 colorectal cancer patients were recruited.

Results: The receiver operating characteristic (ROC) curve analysis showed that the area under the curve (AUC) for all markers tested separately was <0.7. The combination of these modifications showed better diagnostic power (AUC = 0.778 for 8-oxoGua + 8-oxodG)/5hmUra ratio).

Conclusion: Urinary DNA modifications may reflect the oxidative stress/chronic inflammation in colorectal cancer but diagnostic performance for early-detection is moderate.

• DNA damage
• DNA repair
• 5-hmUra
• oxidative stress
• 8-oxoGua

Declaration of interest

The authors report no declarations of interest.

The study was supported by grant from the Polish Ministry of Science and Higher Education (grant number: 2013/09/B/NZ5/00767).