Identification of candidate biomarker mass (m/z) ranges in serous ovarian adenocarcinoma using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling

Abstract

Objective: To differentiate plasma from ovarian cancer and healthy individuals using MALDI-TOF mass spectroscopy.

Materials and methods: MALDI-TOF was used to generate profiles of immuno-depleted plasma samples (89 cancers and 199 healthy individuals) that were fractionated using three types of magnetic beads (HIC8, WCX and IMAC-Cu).

Results: Differentially expressed mass ranges showing >1.5–2-fold change in expression from HIC8 (30), WCX (12) and IMAC-Cu (6) fractions were identified. Cross validation and recognition capability scores for the models indicated discrimination between the classes.

Conclusions: Spectral profiles can differentiate plasma samples of ovarian cancer patients from healthy individuals.

• HIC8
• IMAC-Cu
• MALDI-TOF
• ovarian cancer
• plasma
• WCX

Acknowledgements

We would like to acknowledge Bruker Daltonics, India for their technical support in the proteomics facility.

Declaration of interest

The authors report no declarations of interest. This research was supported by Department of Science and Technology, Government of India [No.SR/S9/Z-08/2010 dated 25.06.2010]. We also thank DST-INSPIRE for supporting the JRF.

Supplementary material available online

Supplementary Tables 1-3