Development of a rapid, cost-effective TaqMan Real-Time PCR Assay for identification and differentiation of Coccidioides immitis and Coccidioides posadasii

Kelly W. Sheff *Translational Genomics Research Institute, Pathogen Genomics Division (TGen North), Flagstaff

Emily R. York *Translational Genomics Research Institute, Pathogen Genomics Division (TGen North), Flagstaff

Elizabeth M. Driebe *Translational Genomics Research Institute, Pathogen Genomics Division (TGen North), Flagstaff

Bridget M. Barker †The University of Arizona, Department of Plant Sciences, Tucson

Steven D. Rounsley †The University of Arizona, Department of Plant Sciences, Tucson

Victor G. Waddell ‡Arizona Department of Health Services, Bureau of State Laboratory Services, Phoenix

Stephen M. Beckstrom-Sternberg **TGen North and Northern Arizona University, Center for Microbial Genetics and Genomics

James S. Beckstrom-Sternberg §Northern Arizona University, Center for Microbial Genetics and Genomics, Flagstaff, Arizona, USA

Paul S. Keim *Translational Genomics Research Institute, Pathogen Genomics Division (TGen North), Flagstaff; §Northern Arizona University, Center for Microbial Genetics and Genomics, Flagstaff, Arizona, USA

David M. Engelthaler *Translational Genomics Research Institute, Pathogen Genomics Division (TGen North), FlagstaffCorrespondence[email protected]

show all

Abstract

Coccidioidomycosis is an infection caused by Coccidioides immitis or C. posadasii. We developed a TaqMan real-time PCR assay that rapidly and accurately differentiates the species. This assay can be used as a tool to improve disease surveillance, increase understanding of the natural history of the infection, and assist in clinical differentiation studies.

Keywords:

Acknowledgements

This research was supported by grants from the Arizona Biomedical Research Commission (# 0816), Science Foundation Arizona (# CAA0242-08) and the Helios Education Foundation.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Development of a rapid, cost-effective TaqMan Real-Time PCR Assay for identification and differentiation of Coccidioides immitis and Coccidioides posadasii

Information for

Open access

Opportunities

Help and information

Development of a rapid, cost-effective TaqMan Real-Time PCR Assay for identification and differentiation of Coccidioides immitis and Coccidioides posadasii

Abstract

Acknowledgements

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature