Abstract
Candida grows on devices producing treatment resistant biofilms. A key tool for the study of biofilms includes an accurate assessment of viable cell growth. This study systematically tested seven techniques, among which the XTT assay provided the most reproducible, accurate, and efficient method for the quantitative estimation of C. albicans biofilms.
Acknowledgements
The authors would like to thank A. Mitchell and C. Nobile for the strains. This work was supported by the National Institutes of Health (RO1 AI073289-01).
Declaration of interest: The authors declare that they have no conflict of interest. The authors alone are responsible for the content and writing of the paper.
This paper was first published online on Early Online on 9 May 2011.