Abstract
During a survey on the incidence of Aspergillus in clinical environments, we found some interesting isolates that were morphologically similar to Aspergillus parasiticus, but differed in the color of the colonies and in the pattern of their conidial ornamentation. In the present study, those isolates were characterized using a polyphasic approach. A phylogenetic analysis was carried out, based on partial fragments of the acetamidase (amdS) and O-methyltransferase (omtS) genes and the internal transcribed spacer (ITS) region of rDNA. This information was combined with a detailed morphological and physiological study that included aflatoxin production and assimilation profiles of different carbon and nitrogen sources. The phenotypic and genotypic results support the proposal of a new species, Aspergillus novoparasiticus, phylogenetically placed in a distinct sister clade to that of A. parasiticus. The former has lobate-reticulate conidia and does not produce aspergillic acid on AFPA or organic acids on CREA, while A. parasiticus has echinulate conidia and produces aspergillic and organic acids. In addition, this new species, as well as A. parasiticus, produces aflatoxins B1, B2, G1 and G2.
Acknowledgements
This work was supported by the Spanish “Ministerio de Educación y Ciencia”, grant CGL 2009-08698/ BOS, co-funding FEDER. S. S. Gonçalves received PhD fellowship from CAPES (SWE 4150-08-2) and CNPq (GM/GD 142051/2007-1). The authors are indebted to Grupo de Micología y Fitopatología, Facultad de Ciencias, Universidad de los Andes for providing the Colombian isolate of A. novoparasiticus.
Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.
This paper was first published online on Early Online on 8 July 2011.