The inter-individual variability outperforms the intra-individual variability of differentially expressed proteins prior and post irradiation in lymphoblastoid cell lines

Abstract

Context: Radio-sensitivity in normal tissue is characterized by heterogeneity throughout the population and the absence of pre-diagnostic biomarkers. Objective: We conducted a proteomic approach to search for radiation characteristic protein regulation. Materials and methods: Cell lines were 10 Gy irradiated and analysed by 2D-DIGE after 24 h. Results were analysed intra- and inter-individually. The principal component analysis and hierarchical clustering was applied to all datasets. Results: Differences in intra-individual spot abundance prior and post irradiation exactly show the separation of sample classes in two groups: sham-irradiated and irradiated. The inter-individual datasets clustered according to the cell line. The intra-individual differences on protein level after gamma-irradiation are very low, compared with the inter-individual differences among cell lines derived from the same tissue. Conclusion: The application of 2-D DIGE may offer a realistic chance for a better molecular characterization of radio-sensitivity and for the discovery of candidate biomarkers.

• 2D-DIGE
• biomarker
• radiation
• radio-sensitivity

Acknowledgements

We like to thank the Institute of Biochemical Plant Pathology of the Helmholtz Center Munich for using the Typhoon scanning unit. This work was financially supported by Federal Ministry of Education and Research (BMBF, Germany), Kompetenzverbund Strahlenforschung FKZ: 03NUK007C and ZISS – FKZ: 02NUK024A.

Supplementary material available online Supplementary figures 1 and 2.