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Abstract
The antioxidative activity of Cleome arabica L. leaf extract was studied toward superoxide anion radical generating systems. The extract at 10 µg/ml has no scavenger effect on superoxide anion radicals generated by the xanthine-xanthine oxidase system, as determined by either the reduction of cytochrome c or pholasin luminescence. Also, this extract, up to 50 µg/ml, did not affect the uric acid production by xanthine oxidase. However, the extract showed a great inhibition activity, in a dose-dependent manner, of neutrophil pholasin luminescence stimulated by formyl-methionyl-leucyl-phenylalanine (fMLP). Indeed, the inhibition of pholasin luminescence was 80.22±2.9%, 90.22 ± 4.0%, and 101.0±1.0% in presence of the extract at concentrations of 1, 2, and 10 µg/ml, respectively. Thereby, it is likely that Cleome arabica leaf extract showed an inhibitory effect on enzyme(s) involved in signaling pathways of fMLP-stimulated neutrophils. Effectively, the extract exerted a significant inhibitory effect (p < 0.05), in a dose-dependent manner, on elastase release by fMLP/cytochalasin B–stimulated neutrophils.