Ethanol Extract of Crataeva nurvala Stem Bark Reverses Cisplatin-induced Nephrotoxicity

Abstract

Generation of free radicals in the kidney cortex plays an important role in the pathogenesis of cisplatin-induced dysfunction of renal proximal tubule cells. Previous studies carried out showed that an alcohol extract of Crataeva nurvala stem bark possessed antioxidant activity in rats, hence the present work aimed at evaluating the possible effect of the alcohol extract of C. nurvala on cisplatin-induced dysfunction model of renal proximal tubule cells by oxidative stress. The alcohol extract was administered orally for ten days at two dose levels of 250 and 500 mg/kg body weight, five days after administration of a single i.p. dose of cisplatin (5 mg/kg). Renal dysfunction was evaluated histologically by light microscopy and biochemically by measuring the concentrations of blood urea nitrogen, serum creatinine, lipid peroxidation, glutathione and catalase activity in the kidney cortex. The results suggest that the plant extract (250 and 500 mg/kg) was effective in significantly altering the indices of cisplatin induced dysfunction of renal proximal tubule cells under oxidative stress by decreasing the concentration of blood urea nitrogen, creatinine and lipid peroxidation. The increased glutathione and catalase activity are indicative of the antioxidant properties of C. nurvala stem bark extract.

Keywords:

• Crataeva nurvala
• cisplatin
• nephrotoxicity
• nephroprotection
• oxidative stress
• antioxidants

Acknowledgements

This study was supported by College of Pharmaceutical Sciences, MAHE, Manipal, India.