In vitro and in vivo anti-Helicobacter/Campylobacter activity of the aqueous extract of Enantia chlorantha

Abstract

The aqueous extract of Enantia chlorantha Oliver (Annonaceae) stem bark, a plant widely used in Cameroon for the traditional treatment of gastritis and stomach problems, was assessed for in vitro and in vivo anti-Helicobacter/Campylobacter properties using the well diffusion assay, agar dilution assay, and killing rate determination. The in vitro activity was dose-dependent, and the same antimicrobial parameters (MAQ = 0.63 mg; MIC = 0.39 mg/mL; MBC = 1.56 mg/mL; ET₁₀₀ = 8 h) were obtained for both H. pylori and C. jejuni/coli. When the plasma active principle concentration equivalence was determined in vitro using plasma from rats exposed to a single dose (3000 mg/kg) of the extract, the peak absorption of E. chlorantha active principle against H. pylori occurred at 2 h. Plasma activity was nil 8 h after extract administration. The in vivo H. pylori eradication potency of the extract was assessed using mice infected with H. pylori. Antral mucus sample cultures from mice treated with E. chlorantha extract (500 and 1000 mg/kg for 3 days) did not yield any growth. The results suggest that in addition to its in vitro activity, E. chlorantha water extract also possesses in vivo antibiotic effects against H. pylori.

Keywords:

• Enantia chlorantha
• Helicobacter pylori
• in vivo
• in vitro activity