Antimicrobial activities of neo- and 1-epineo-isoshinanolones from Plumbago zeylanica roots

Abstract

Context: The roots of Plumbago zeylanica Linn. (Plumbaginaceae) are reputed to have a wide spectrum of therapeutic properties in the Ayurvedic system of medicine. They are useful in curing many ailments such as skin diseases, diarrhea, plague and leprosy.

Objective: The study was aimed at isolating, separating and evaluating the antimicrobial properties of compounds such as neoisoshinanolone and 1-epineo-isoshinanolone from the roots of P. zeylanica.

Materials and methods: The crude petroleum ether extract of roots of P. zeylanica was subjected to repeated chromatographic techniques to separate compounds 2 and 3 along with plumbagin. Structure elucidation was carried out using nuclear magnetic resonance (NMR), infra red (IR) and mass spectroscopy. The serial dilution method was used to test antimicrobial activities and their minimum inhibitory concentration (MIC) expressed in µg/mL.

Results: 1-Epineo-isoshinanolone is more active with a MIC of 12.5-25 µg/mL whereas neoisoshinanolone has recorded a MIC of 50-100 µg/mL. The activities are compared with plumbagin (0.78-3.13 µg/mL) and standards streptomycin for bacteria and nystatin for fungi.

Discussion: Earlier researchers have established the presence of plumbagin in the roots of P. zeylanica and its antimicrobial activities. The structure elucidation of two more biologically active biogenetic precursors along with their activities in the root extracts has been established for the first time in the present study.

Conclusion: The root extract of P. zeylanica possesses good antimicrobial activity, which suggests its therapeutic use in the Ayurvedic system of medicine to cure skin diseases.

Keywords::

• Antimicrobial activity
• neoisoshinanolone
• 1-epineo-isoshinanolone
• Plumbago zeylanica
• plumbagin

Acknowledgements

The authors are grateful to J. S. Yadav, Director IICT, for encouragement and cooperation and Prof. Prabhakar Malve, Taxanomist, Osmania University, Hyderabad for authentication of the plant material.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.