Antinociceptive and anti-inflammatory effects of Costus spicatus in experimental animals

Abstract

Context: Costus spicatus Swartz (Costaceae), commonly called “cana-do-brejo’” in Brazil’s northeast, is a medicinal plant found in wet coastal forests. In folk medicine an infusion of the aerial parts is taken to treat inflammation and pain.

Objective: The methanol extract obtained from the leaves of Costus spicatus (MECs) was evaluated for antinociceptive and anti-inflammatory activities.

Methods: Analgesic and anti-inflammatory activities were studied by measuring nociception through acetic acid, formalin, and hot-plate tests, while inflammation was induced by carrageenan. All experiments were conducted with experimental animals.

Results and discussion: Following oral administration, MECs (100, 200, and 400 mg/kg) significantly reduced the number of writhes (52.8, 43.1, and 55.3%, respectively) in the writhing test and the number of paw licks during phase 1 (61.9, 54.1, and 92.1%) and phase 2 (62.5, 82.9, and 98.1%, all doses) during the formalin test when compared to the control group animals. The reaction time during the hot-plate test was increased significantly and was dose-dependent, whereas pretreatment with naloxone rigorously reduced the analgesic potential of MECs, which suggested participation of the opioid system in the modulation of pain induced by MECs. Such results were unlikely to be provoked by motor abnormality, as MECs-treated mice did not exhibit any performance alteration during the Rota-rod test. The administration of 200 and 400 mg/ kg (i.p.) of MECs exhibited an anti-inflammatory effect during the carrageenan test, which was based on interference with inflammatory mediator synthesis.

Conclusion: We conclude that MECs has antinociceptive and anti-inflammatory activities in rodents.

Keywords::

• Costus spicatus
• inflammation
• nociception
• acetic acid
• carrageenan
• formalin
• hot-plate

Acknowledgements

We thank Mr Osvaldo Andrade Santos for technical support. This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq, Fundação de Apoio à Pesquisa e à Inovação Tecnológica do Estado de Sergipe – FAPITEC-SE, CAPES, Ministério da Saúde, and Secretaria Estadual de Saúde – SES/SE, Brazil.

Declaration of interest

Grant Edital 06/2007, to one of the authors (Márcio R.V. Santos), Project Coordinator.