Study on hemostatic activities of the rhizome of Paris bashanensis

Abstract

Context. Paris bashanensis Wang et Tang (Liliaceae) is widely used in traditional Chinese medicine for the treatment of injuries, fractures and hemorrhage in Hubei and Sichuan Province.

Objective: The n-BuOH extract of Paris bashanensis was investigated for hemostatic activity and chemical constituents in order to provide a basis for the application in folk use.

Materials and methods: The n-BuOH extract of P. bashanensis was divided into three eluents (30, 50 and 70% EtOH) by macroporous adsorptive resin D101. The bleeding time of breaking tail hemostasis and clotting time of capillary and slide method in mice were used extensively to screen the hemostasis properties after repetitive administration of these three fractions (100 mg/kg, 50 mg/kg) for 5 days (total of 5 times, once per day). The chemical compounds were analyzed by HPLC-UV.

Results: The inhibition rates in the bleeding time of 70, 50 and 30% n-BuOH ext. were 45, 32 and 21%, respectively. Using the slide method the decreasing rate of the clotting time of 70, 50 and 30% n-BuOH ext. were 71, 65 and 32% and in the experiment of capillary method, the inhibition rates were 43, 31 and 24%, respectively. A total of 70% n-BuOH ext. showed a high content of the pennogenin-type saponins by HPLC-UV.

Discussion and conclusions: The 70% n-BuOH ext. of P. bashanensis was found to contain high levels of pennogenin saponins, which may lead to a higher hemostatic activity. Combined with the hemostatic test, P. bashanensis could be used as a resource of hemostatic drug.

• Blood clotting time
• HPLC-UV
• pennogenin saponins
• steriodal saponins
• tail bleeding time

Acknowledgement

Financial support from the Doctoral Scientific Fund Project of the Ministry of Education of China (Grant No. 20090032110059) is gratefully acknowledged.