Efficient protocol for rapid Aloe vera micropropagation

Abstract

Context: Aloe vera Linn. (Liliaceae) is a medicinal plant and has a number of curative properties. Vegetative propagation has not enough potential for supplying market demand. However, via in vitro propagation makes possible the mass production of Aloe plants.

Objective: The current study was conducted to investigate growth regulators’ effects on proliferation of A. vera.

Materials and methods: In this study, for comparison of plant growth regulators’ effects on proliferation, the shoot tips and auxiliary buds of A. vera were cultured in the Murashige and Skoog (MS) medium. Rooted plantlets were transferred to garden soil, compost, and sand in the proportion of 1:1:1, respectively, after hardening.

Results: The maximum number of shoots was obtained on the medium supplemented with 1 mg/L IAA + 4 mg/L BAP and 0.2 mg/L IAA + 0.8 BAP mg/L. Rooting was also achieved in the same media composition proliferation of shoot. The acclimatized plants showed 100% of survival. The regenerated plants looked healthy, and they were morphologically similar to that of stock plants.

Conclusion: These results suggest that in vitro culture may be used as a technique for rapid propagation of A. vera.

• In vitro culture
• medicinal plant
• plant growth regulators
• proliferation
• shoot tip