Abstract
Context: In Kenya, most people use traditional medicine and medicinal plants to treat many diseases including malaria. To manage malaria, new knowledge and products are needed. Traditional herbal medicine has constituted a good basis for antimalarial lead discovery and drug development.
Objectives: To determine in vivo antimalarial activity and brine shrimp toxicity of five medicinal plants traditionally used to treat malaria in Msambweni district, Kenya.
Materials and methods: A 0.2 ml saline solution of 100 mg/kg aqueous crude extracts from five different plant parts were administered orally once a day and evaluated for their in vivo chemosuppressive effect using Plasmodium berghei berghei-infected Swiss mice for four consecutive days. Their safety was also determined using Brine shrimp lethality test: Grewia trichocarpa Hochst ex A. Rich (Tiliaceae) root, Dicrostachys cinerea (L) Wight et Am (Mimosaceae) root, Tamarindus indica L. (Caesalpiniaceae) stem bark, Azadirachta indica (L) Burn. (Meliaceae) root bark, and Acacia seyal Del. (Mimosaceae) root.
Results: Parasitaemia was as follows: A. indica, 3.1%; D. cinerea, 6.3%; T. indica, 25.1%; A. seyal, 27.8%; and G. trichocarpa, 35.8%. In terms of toxicity, A. indica root bark extract had an LC50 of 285.8 µg/ml and was considered moderately toxic. T. indica stem bark extract and G. trichocarpa root extract had an LC50 of 516.4 and 545.8 µg/ml, respectively, and were considered to be weakly toxic while A. seyal and D. cinerea root extracts had a LC50 >1000 µg/ml and were, therefore, considered to be non-toxic.
Discussion and conclusion: All extracts had antimalarial activity that was not significant compared to chloroquine (p ≥ 0.05). No extract was toxic to the arthropod invertebrate, Artemia salina L. (Artemiidae) larvae, justifying the continued use of the plant parts to treat malaria.
Acknowledgements
The authors are indebted to the community of Msambweni district for sharing their knowledge and time with them; to Mr. Mutundu Gabriel of Centre for Traditional Medicine and Drug Research laboratories-Kenya Medical Research Institute, Nairobi and Mr. Joseph Gichuki Nderitu of Department of Public Health, Pharmacology and Toxicology, University of Nairobi for their technical assistance and to Mr. Kimeu Musembi, a taxonomist at the University of Nairobi Herbarium, Nairobi, who identified the study plants.
Declaration of interest
The authors declare no conflicts of interest. The authors are grateful to Regional Initiative in Science and Education-African Natural Product Network (RISE-AFNNET) for supporting this study.