Analysis of IgG4-positive clones in affected organs of IgG4-related disease

Abstract

Objective: We investigated class switch reaction (CSR) in affected organs and evaluated whether the same or genetically related clones exist in IgG4-RD.

Methods: We studied three patients with IgG4-RD. Total cellular RNA was extracted from salivary glands and peripheral blood and lung tissue. Activation-induced cytidine deaminase (AID) and immunoglobulin heavy chain third complementarity determining region (IgVH-CDR3) of IgM and IgG4 were detected by reverse transcription polymerase chain reaction (RT-PCR). We analyzed the clonal relationship of infiltrating IgG4-positive cells, as compared with IgM. We determined the existence of common clones among organs and patients.

Result: AID was expressed in salivary glands of all patients and lung tissue in one. Closely related IgVH-CDR3 sequences in infiltrating IgG4-positive cells were detected in salivary glands and lung tissue. Identical IgVH-CDR3 sequence between IgM and IgG4 in salivary glands was detected in one patient, indicating CSR in salivary glands. Identical IgVH-CDR3 sequences of IgG4-positive cells were detected between salivary glands and peripheral blood in two patients. Four identical sequences of IgVH-CDR3 existed between patients. Interestingly, one of the four sequences was detected in all patients.

Conclusion: Our results demonstrate the existence of common antigen(s) shared by patients with IgG4-RD.

Keywords:

• CDR3
• Common antigen
• IgG4-related disease

Acknowledgments

We thank Mr. John Gelblum for his critical reading of the manuscript.

Conflict of interest

This work was supported partially by the Research Program of Intractable Diseases of the Ministry of Health, Labor, and Welfare of Japan, and by JSPS KAKENHI Grant Number 26461487.